American Journal of Phytomedicine and Clinical Therapeutics (AJPCT)

Online ISSN: 2321-2748

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Publisher: TIba pharmaceuticals Pvt. Ltd.
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Antiarthritic Evalution of Crateva religiosa Extracts

Author(s): Shyamalendu Tripathy, Debashis Pradhan, Bimala Tripathy
Abstract
| Pages: 370-377
The anti-arthritic potential of Crateva religiosa hook & frost belonging to family Capparidaceae were evaluated by taking complete Freund’s adjuvant (CFA) induced model. Arthritis was induced by injecting 0.1ml of complete Freund’s adjuvant below the plantar aponeurosis of the right hind paw. Treatment with the extracts and standard started on the day of induction of CFA and continue up to 28 days. In this study both the alcoholic and aqueous extracts significantly (p

Screening of Biological Activities (Antioxidant, Antibacterial and Antitumor) of Nerium oleander Leaf and Flower Extracts

Author(s): Pegah Namian, Taravat Talebi, Karimollah Ghasemi Germi, Fahmideh Shabani
Abstract
| Pages: 378-384
Oleander (Nerium oleander L. Apocyanaceae) is an evergreen shrub, widely use for medicinal purposes. The objective of this work is to investigate the antioxidant, antibacterial and cytotoxic effects of the plant leaves (L) and flowers (F) extracts. These activities had been evaluated by DPPH, agar disc diffusion, agar dilution and MTT, respectively. Our results showed that methanol extracts (L & F) have high antioxidant activity with RC50 value of 0.27, 0.2mg/mL respectively, dichloromethane (DCM) and methanol extracts (L & F) showed strong antibacterial activity against both gram negative and gram positive bacteria. MTT assay exhibited that dichloromethane extracts (L & F) have high cytotoxic effects against T47D, HepG-2 and K562 cell lines with IC50 value of 57.77, 233.42μg/mL, 55.90, 108.31μg/mL, 70.03, 102.31μg/mL, respectively.

Effect of Leaves of Carissa spinarum Linn. on Blood Glucose and Lipid Profile in Alloxan Induced Diabetic Rats

Author(s): Amreen Fatima, Prem Prakash Singh, Raghuveer Irchhaiya, Parul Agarwal
Abstract
| Pages: 385-394
Objective: The aim of the study is to analyze the antidiabetic effect of oral administration of acetone extract of Carissa spinarum (C. spinarum) leaves on blood glucose, haemoglobin, plasma insulin and lipid peroxidation to alloxan-induced diabetic rats. Method: Diabetes mellitus was induced by injection of alloxan (150mg/kg body weight), via the intraparitoneally. The extract was administered orally at 200, 400 and 600 mg/kg body weight (both to normal and diabetic rats), and glimepiride at 2g/kg body weight. Results: Acute toxicity study shows acetonic extract is safe or non toxic even at the dose of 2000 mg/kg body weight. The effect of the extract at a dose of 600 mg/kg body weight was highly significant than 200mg/kg, 400 mg/kg and Glimepiride 2 g/kg body weight. Administration of the extract at various doses and glimepiride tends to significantly bring the FBG level to normal. Urine sugar also tends to decrease at different doses of acetonic extract of C. Spinarum, The effect of C. spinarum extract on Cholesterol, Triglyceride, LDL and VLDL values increased significantly in diabetic rats, whereas HDL values decreased significantly in diabetic rats. C. spinarum extract treatment decreased the Cholesterol, Triglyceride, LDL and VLDL values and increased the HDL values significantly in diabetic treated rats. Conclusions: Our results suggest that acetonic extract of C. spinarum exhibit antihyperglycemic as well as antihyperlipidaemic effects on alloxan-induced diabetic rats.

Development and Validation of Reversed- Phase High Performance Liquid Chromatographic Method for Estimation of Dexketoprofen Trometamol in Bulk and Tablet Dosage Form

Author(s): Kadam Archana, Patil Vikas
Abstract
| Pages: 395-400
The Literature survey indicates several methods for the determination of Dexketoprofen trometamol. So an attempt was made to develop and validate a simple, precise, accurate, and economical RP-HPLC method as per ICH guidelines for the estimation of Dexketoprofen trometamol in bulk and pharmaceutical dosage forms. A simple reverse phase HPLC method was developed for the determination of Dexketoprofen trometamol present in pharmaceutical dosage forms. C18 (4.6ID x 250mm) in an gradient mode with mobile phase Acetonitrile: Methanol (25:75) was used. The flow rate was 0.9ml/ min and effluent was monitored at 262nm. The retention time was 2.9min for Dexketoprofen trometamol. The linearity ranges were found to be 30-70μg/ml.

Development and Validation of UV/Visible Spectrophotometric Method for the Estimation of Rifapentine in Bulk and Pharmaceutical Formulations

Author(s): Ansari Tahir, Kalkotwar R.S, Jeevan Naikwade, Afsar Shaikh
Abstract
| Pages: 401-407
A simple, accurate, precise and sensitive UV/visible spectrophotometric method were developed for the determination of Rifapentine in bulk and pharmaceutical dosage form. The solvent used was methanol and the wavelength corresponding to maximum absorbance of the drug was found at 478nm. Drug obey’s Beers law in the concentration range of 10- 60ug/ml with correlation coefficient 0.9997. The linear regression equation obtained by least square regression method was y=0.0107x-0.0333, where y is the absorbance and x is the concentration of the pure drug solution. The method was validated for several parameters like accuracy, precision as per ICH guidelines. The values of relative standard deviation and % recovery were found to be satisfactory, indicating that the proposed method is precise and accurate and hence can be used for the routine analysis of Rifapentine in bulk and pharmaceutical formulation.

Cardioprotective Effect of the Methanolic Extract of Boiled Peanut (Arachis hypogaea) on Isoproterenol Induced Myocardial Infarction in Albino Rats

Author(s): Karthik Mohan, Karthika
Abstract
| Pages: 408-415
Epidemiological studies revealed that Indians are at greater risk of getting myocardial infarction. On the other hand, India sports the highest per capita consumption of fresh peanuts globally, standing at 6.3kg in 2010. The boiled peanuts (Arachis hypogaea) is said to possess a bioactive phytoprinciple, resveratrol, which is said to be a potent cardioprotective agent. Hence this study was designed to investigate the cardio protective effect of the methanolic extract of boiled peanuts (Arachis hypogaea) against isoproterenol induced experimental myocardial infarction in albino rats. Wistar albino rats were pretreated with the methanolic extract of A. hypogaea (33mg / kg. of body weight, orally) for 15 days and then induced with isoproterenol (ISO 30 mg/kg of body weight, sub-cutaneously for 2 consecutive days). On 18th day, blood was collected by cervical decapitation under anesthesia. Heart was removed for histopathological studies. Serum samples were used to estimate total cholesterol, triglycerides, HDL, LDL. The enzyme markers lactate dehydrogenase, SGOT and SGPT were estimated. In isoproterenol, induced rats levels of cholesterol, TG, LDL were increased significantly with a decrease in HDL level and the enzyme markers like LDH, SGOT, SGPT levels were increased. The pretreated group animals with A. hypogaea showed decreased marker enzymes level, cholesterol, TG, LDL and increased HDL level. The results suggested that the methanolic extract of Arachis hypogaea possess cardioprotective effect on experimentally induced myocardial infarcted albino rats. The activity might be because of the high content of resveratrol present in the boiled peanuts.

Phytochemical Screening and Evaluation of In vitro Antioxidant Activity of Viburnum punctatum buch-ham. Ex d.don

Author(s): Ilango.K, Renjith Alex.A
Abstract
| Pages: 416-423
The present study was to estimate the preliminary phytochemical screening and in vitro anti-oxidant activity of aerial part extracts of Viburnum punctatum by using solvents like Petroleum ether, Chloroform, Methanol and Water. Preliminary phytochemical analysis reveals the presence of alkaloids, glycosides, flavonoids, phenolic compounds, proteins, phytosterols and saponins. The chloroform and methanol extract were screened for its potential antioxidant activity by DPPH radical scavenging activity, Hydroxyl radical scavenging activity, Nitric oxide scavenging activity, Reducing power activity and Superoxide free radical scavenging activity using Ascorbic acid as a standard. The results of these five methods anti-oxidant study indicate ta concentration dependent antioxidant activity of various concentrations of 50μg/ml, 100μg/ml and 200μg/ml of the extract respectively. The DPPH radical scavenging activity and other radical scavenging activities of the extract was increased with increasing concentration. The methanol extract exhibited better anti-oxidant activity than chloroform extract for all the five in vitro anti-oxidant activity screened.