Ports and Maritime organization that is in charge of handling the highest volume of container operations in Iran plays a very important role in economy and trade of that country This organization is also trying to maintain a high level of responsiveness to achieve agility and to remain competitive in the global marketplace, and so it needs to consider Business Process Re-engineering (BPR). Application of business process re-engineering is the way for successful re-engineering of processes. The present study is aimed at investigating the effect of business process re-engineering factors on organizational agility using path analysis in Ports and Maritime Organization of Iran. BPR factors are operationalized by cultural factors, communications, methodology, project management, strategic alignment, information technology, leadership, empowerment, and performance management.Initially the theoretical principles were discussed. The data gathered in this stage provided us with the possibility to present a conceptual framework for the study. Subsequently, through interviewing with experts, some indices for evaluating the variables in the model were identified. On the following stage, a questionnaire was developed. The questionnaire included 55 items, based on a 5-point Likert scale. 120 questionnaires were distributed to marine, training, assessment, financial, and information technology (IT) divisions of Ports & Maritime Organization. Using path analysis, with partial standardized regression coefficient, the priority and grade of every independent variable could be defined based on affecting the depended variable. The results of the research revealed that leadership and empowerment variables had the most effect on organizational agility than other variables.